Rabbit Anti-PHAP I, CT (Putative HLA DR-associated Protein I, PHAPI, Acidic (Leucine rich) Nuclear Phosphoprotein 32 Family Member A, ANP32A, C15orf1, Cerebellar Leucine-rich Acidic Nuclear Protein, Inhibitor 1 of Protein Phosphatase 2A, I1PP2A, LANP, Mapmodulin, MAPM, MGC119787, MGC150373, Potent Heat Stable Protein Phosphatase 2A Inhibitor I1PP2A, PP32) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
PHAP I is one of the known isoforms of PHAP. PHAP I plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumor suppression. It is expressed in a variety of tissues with highest expression in kidney and skeletal muscle.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: detects a band at ~32kD in Raji cell lysate Immunohistochemistry (Paraffin): 2ug/ml requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human PHAP I.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PHAP I. Has no cross-reaction to PHAP I2a and PHAP III. Species Crossreactivity: mouse and rat
