Rabbit Anti-PHLPP (PH Domain and Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat-containing Protein Phosphatase, PHLPP1, KIAA0606 Protein, Pleckstrin Homology Domain-containing Family E Protein 1, PLEKHE1 Protein, Suprachiasmatic Nucleus Circadian Oscillatory Protein, SCN Circadian Oscillatory Protein, SCOP, Suprachiasmatic Nucleus Circadian Protein) (PE)
PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1,000 Optimal dilutions to be determined by the researcher.
Positive Control
Human brain tissue lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PHLPP1. Species Crossreactivity: mouse and rat.
