Mouse Anti-Phosphatase, Alkaline, Liver, Bone, Kidney (Akp2, ALPI, ALPL)
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody can be used to monitor the expression of the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by Flow Cytometry.
Applications
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
2102Ep human Embryonal Carcinoma Cells. Epitope: Liver/Bone/Kidney Alkaline Phosphatase.
Form
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Does not crossreact with other isoforms of alkaline phosphatase. Species Crossreactivity: porcine, feline, tiger, rabbit, higher primates (chimpanzee, gorilla, orangutan, gibbon), and New World monkeys (owl monkey, squirrel monkey). No crossreactivity has been observed with bovine, canine, goat, guinea pig, hamster, rat, mouse, sheep and Old World monkeys (baboon, Rhesus monkeys, mangabey, mandrill, Celebese black ape, African green monkey).
