P4071-78A2
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_005019Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PIP5K2A, CT (Phosphatidylinositol-5-phosphate 4-kinase Type-2 alpha, 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha, Diphosphoinositide Kinase 2-alpha, PIP5KIII, Phosphatidylinositol-5-phosphate 4-kinase Type II alpha, PI(5)P 4-kinase Type II alpha, PIP4KII-alpha, PtdIns(4)P-5-kinase B Isoform, PtdIns(4)P-5-kinase C Isoform, PtdIns(5)P-4-kinase Isoform 2-alpha, PIP4K2A, PIP5K2)
Phosphatidylinositol-4,5-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. TPIP5K2A is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. The protein is a member of the phosphatidylinositol-4-phosphate 5-kinase family.
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human PIP5K2A (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human PIP5K2A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Rozenvayn, N., et al., J. Biol. Chem. 278(10):8126-8134 (2003). 2.Boronenkov, I.V., et al., J. Biol. Chem. 270(7):2881-2884 (1995). 3.Loijens, J.C., et al., Adv. Enzyme Regul. 36, 115-140 (1996). 4.Divecha, N., et al., Biochem. J. 309(Pt 3), 715-719 (1995).USBio References
No references available