Rabbit Anti-Phosphodiesterase 4B (PDE4B) (MaxLight 490)
MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Of the 11 families of phosphodiesterase (PDEs) identified, PDE4, PDE7, and PDE8 are specific for cAMP. However, isoforms of the PDE4 family often account for most of the cAMP-hydrolyzing activity of a cell. Four genes (PDE4A, B, C and D) make up the PDE4 family. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In the CNS, PDE4B subtype variants are expressed in varying ratios. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants.
Applications
Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilution; Immunoprecipitation: 7ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the N-terminal region of human and rat PDE4B protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Reacts with the human and rat PDE4B protein. On Western blots, it identifies a single band at ~83kD. Bands of unknown origin may be observed at ~160 and ~260kD. Reactivities have been confirmed with NG96 human neuroblastoma lysate and rat brain homogenate.
