Technical Data

P4073-14D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_620061
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Phosphoglycerate Kinase 2, NT (PGK2, dJ417L20.2, PGKB, PGKPS, Phosphoglycerate Kinase Testis Specific)
EC=2.7.2.3

PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis suggests that it exists as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human PGK2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human PGK2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Zhang, L.P., et al., Biol. Reprod. 60(6):1329-1337 (1999). 2.McCarrey, J.R., et al., Dev. Genet. 19(4):321-332 (1996). 3.Blanche, H., et al., Genomics 9(3):420-428 (1991). 4.McCarrey, J.R., et al., Nature 326(6112):501-505 (1987). 5.Michelson, A.M., et al., J. Biol. Chem. 260(11):6982-6992 (1985).
USBio References
No references available
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