Technical Data

P4074-40-HRP
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Conjugate
HRP
Isotype
IgG
Clone Number
0.T.171 (K-32-3, K-92-3)
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Bo Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Mouse Anti-Phospholipase C, beta 1 (PLCB1, KIAA0581, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, Phospholipase C-beta-1) (HRP)
Applications
Suitable for use in Immunoprecipitation/Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunoprecipitation/Western Blot: 5ug immunoprecipitated PLC b-1 from a bovine brain membrane preparation. The immunoprecipitation procedure was followed by an Western Blot using 5ug/ml of P4074-40 and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Mixed monoclonal antibodies raised against purified bovine PLCβ-1
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes bovine PLC b-1 at 150kD. Also recognizes the 100kD and 41kD fragments of PLC b-1. Species Crossreactivity: rat, mouse and human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Suh, P.G., et al., J. Biol. Chem. 263: 14497–14504, 1988. 2. Rhee, S.G., et al., Proc. Natl. Acad. Sci. USA 88: 5453, 1991. 3. Meisenhelder, J., Cell 57: 1109–1122, 1989. 4. Ryu, S.H., J. Biol. Chem. 262: 12511–12518, 1987. 5. Liu, et al, J Biol. Chem. 271: 355, 1996. 6. Kind, P.C., et al., J. Neurosci. 17: 1471-1480, 1987
USBio References
No references available
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