P4074-45
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IP WBCrossreactivity
Hu Mo RtGene ID
5336Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Phospholipase C, gamma 2 (PLCg2)
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs: PLC beta, PLC gamma, PLC delta and PLC epsilon, have been identified. The PLC beta subfamily includes four members, PLC beta1-4. All four members of the subfamily are activated by alpha- or beta-gamma-subunits of the heterotrimeric G-proteins (2,3).Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of serine 1105 by PKA or PKC inhibits PLC beta3 activity (4,5). Serine 537 of PLC beta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLC beta3. PLC gamma is activated by both receptor and nonreceptor tyrosine kinases (6). PLC gamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLC gamma1 on tyrosine residues 771, 783 and 1245 (7). Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLC gamma1 (8). PLC gamma2 is engaged in antigen-dependent signaling in B cell and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at tyrosines 753, 759, 1197 and 1217 is correlated with PLC gamma2 activity (9,10).
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000; Band at ~150kD Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding the C-terminus of human PLC gamma 2.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Available without azide, BSA and glycerol. See P4074-45B.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human total PLC gamma2 protein at 150kD. Does not crossreact with PLC gamma1. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Singer, W.D., et al., Annu. Rev. Biochem. 66: 475–509 (1997). 2. Smrcka, A.V., et al., Science 251: 804–807 (1991). 3. Taylor, S.J., et al., Nature 350: 516–518 (1991). 4. Yue, C., et al., J. Biol. Chem. 273: 18,023–18,027 (1998). 5. Yue, C., et al., J. Biol. Chem. 27: 30,220–30,225 (2000). 6. Margolis, B., et al., Cell 57: 1101–1107 (1989). 7. Kim, H.K., et al., Cell 65: 435–442 (1991). 8. Wang, Z., et al., Mol. Cell. Biol. 18: 590–597 (1998). 9. Watanabe, D., et al., J. Biol. Chem. 276: 38,595–38,601 (2001). 10. Ozdener, F., et al., Mol. Pharmacol. 62: 672–679 (2002).USBio References
No references available