Technical Data

P4074-52
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
IHC
Crossreactivity
Hu Mo
Accession #
NP_002654
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PLD2 (Phospholipase D2, PLD 2, hPLD2, EC=3.1.4.4, Choline Phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing Phospholipase D2) (Not for Export EU)

PLD2 is a peripheral membrane protein belonging to phospholipase D family with a PH domain, two PLD phosphodiesterase domains and a PX (phox homology) domain that plays a regulatory rather than catalytic role. PLD2 catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline. PLD2 may function in regulated secretion, signal-induced cytoskeletal regulation and/or endocytosis, transcriptional regulation, and cell cycle control. It is usually stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and activated by the ADP-ribosylation factor-1 (ARF-1). PLD2 is activated by agonist stimulation of both tyrosine kinase and G protein-coupled receptors and is also known to interact with EGFR and PIP5K1A. PLD2, localized to plasma membrane caveolae, is ubiquitously expressed in most tissues.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (formalin fixed paraffin embedded): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa199-216 DLGRKGLEGMIRKRSGGH of human PLD2.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Purity
Serum
Specificity
Recognizes human PLD2. Species Crossreactivity: chimpanzee. Species sequence homology: equine, mouse, opossum, pufferfish, rhesus monkey, canine, bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Liscovitch, M. et al. Biochem. J. 345:401-415 (2000). 2. Lopez, I. et al. J. Biol. Chem. 273:12846-12852 (1998). 3. Park, SH. et al. Cytogenet. Cell Genet. 82:225 (1998).
USBio References
No references available
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