Mouse Anti-Phosphoserine
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated proteins, peptides, and antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Applications
Suitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot (Indirect): 1:500-1:1000 Indirect ELISA: 1:8000-1:16,000 Note: Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Optimal dilutions to be determined by researcher.
Positive Control
Rat brain cortex and extract at ~50kD.
Hybridoma
Mouse myeloma cells with splenocytes from immunized mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Phosphoserine conjugated to KLH.
Form
Supplied as a liquid, 15mM sodium azide.
Specificity
Recognizes phosphorylated serine both as free amino acid or when coupled to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody because of steric hindrance of the recognition site. Species Crossreactivity: rat
