Technical Data

P4186-03-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
3H2837A (U5)
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Bo Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Frozen Western Blot: 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to the bovine p85 alpha subunit of PI 3 kinase, expressed in Baculovirus.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase. Species Crossreactivity: human, mouse, rat and monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Reif, K. et al. (1993) Divergent regulation of phosphatidylinositol 3-Kinase P85 alpha and P85 beta isoforms upon T cell activation. J. Biol. Chem. 278: 10780-10788.
USBio References
No references available
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