Technical Data

P4186-60F-APC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Rabbit Anti-Pim1, CT (PIM-1, Oncogene PIM1, Oncogene PIM-1, Pim1 Kinase, Pim-1 Kinase, Pim1 Oncogene, Pim-1 Oncogene, PIM, Proto-oncogene Serine/threonine-protein Kinase Pim-1, Proto-oncogene Serine/threonine Protein Kinase Pim1) (APC)
Proviral Integration Site 1, EC=2.7.11.1

PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.

Applications
Suitable for use in FLISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 374-404 in the C-terminal region of human Pim1. |Species Sequence Homology: bovine
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Pim1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Pasqualucci, L., et al., Nature 412(6844):341-346 (2001). 3. Koike, N., et al., FEBS Lett. 467(1):17-21 (2000). 4. Reeves, R., et al., Gene 90(2):303-307 (1990). 5. Telerman, A., et al., Mol. Cell. Biol. 8(4):1498-1503 (1988). 6. Jian Chen, et al. Am. J. Pathol. 2009; 175:400-411. (Applications: WB)
USBio References
No references available
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