Technical Data

P4186-63C
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Accession #
NP_001001852
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PIM3, CT (Pim-3, Pim-3 Oncogene, Serine/threonine Protein Kinase Pim-3)
EC=2.7.11.1

PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 259-288 in the C-terminal region of human PIM3, UniProt Accession #Q86V86. |Species Sequence Homology: rat
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human PIM3. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Mikkers, H., et al., Mol. Cell. Biol. 24(13):6104-6115 (2004). 2. Eichmann, A., et al., Oncogene 19(9):1215-1224 (2000). 3 .Lisa S. Chen, et al. Blood, Nov 2009; 114: 4150 - 4157. 4. Lisa S. Chen, Sanjeev Redkar, David Bearss, William G. Wierda, and Varsha Gandhi, et al. Blood. published 4 September 2009, 10.1182/blood-2009-03-212852. (Applications: WB)
USBio References
No references available
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