Rabbit Anti-Pirh2 (p53-induced RING-H2 Protein, hPirh2, Androgen Receptor N-terminal Interacting Protein, ARNIP, CH-rich Interacting Match with PLAG1, CHIMP, E3 Ubiquitin-protein Ligase Pirh2, Ring Finger and CHY Zinc Finger Domain Containing 1, RCHY1, RING Finger Protein 199, RNF199, Zinc Finger Protein 363, ZNF363)
EC=6.3.2.-, DKFZp586C1620, PRO1996
RING finger and CHY1 zinc finger domain containing protein 1 (RCHY1) is a newly identified RINGH2- type protein-ubiquitin E3 ligase that is expressed as multiple isoforms generated through alternative splicing of mRNA transcripts (1,2). Notably, RCHY1 is a p53-regulated gene and mutiple studies have shown that RCHY1 directly binds p53 to promote its ubiqutin-dependent proteasomal degradation, ultimately leading to repression of p53 growth suppressive transcriptional activity (1,3). RCHY1 is also likely to promote deregulated cell proliferation by targeting the CDK inhibitor, p27/KIP1, for proteasomal degradation (4). In agreement with its growth promoting activity, RCHY1 overexpression hs been linked to cancer of the lung (5,6) and prostate (7).
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human RCHY1 protein isoform1. Species sequence homology: monkey (100%)
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of total human RCHY1. Species Crossreactivity: mouse. This antibody does not detect RCHY1 dimers.
