Plakophilin-3 is a novel 87kD Armadillo-like (Arm) protein, which is characterized by a series of repeats of 42-45 a.a., originally described in the Drosophila protein Armadillo. Plakophilin-3 is a member of the p120ctn/plakophilin subfamily, which includes plakophilin-1, plakophilin-2, the ARVCF gene product and d-catenin. This protein is similar to plakophilin-1 and plakophilin-2. Other Arm proteins include b-catenin and a-catenin. All these proteins are part of the cell-cell adhesion complex . On Western Blot analysis, plakophilin-3 was expressed in epithelial cell lines but not in foreskin fibroblasts and sarcomaderived cell lines. By Immunofluorescence, plakophilin-3 was colocalized with desmoglein in desmosomes of epithelial cells

Suitable for use in FLISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. Other applications have not been tested.

FLISA: 0.1-1ug/ml Immunoprecipitation: 10ug/IP reaction Immunohistochemistry: 10ug/ml Immunoflluorescence: 1-3ug/ml Western Blot: 1-3ug/ml Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2% Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to the Armadillo domain of human Plakophilin-3.

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography

Recognizes the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.