Technical Data

P4256-25L
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
9G247 (174/2)
Grade
Affinity Purified
Applications
FC IF IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-PLVAP (Plasmalemma Vesicle-associated Protein, Fenestrated Endothelial-linked Structure Protein

PLVAP is a 60kD type II transmembrane glycoprotein which tends to form homodimers. PLVAP is widely expressed in the vasculature of normal tissues. The vascular staining pattern of PLVAP is quite unique among the endothelial cell antigens, since expression of other antigens (like ICAM-1, P-selectin, E-selectin or VCAM-1) is not limited to vascular endothelial cells. PLVAP expression in the vascular endothelial cells of the CNS is lost in association with formation of an intact blood-brain barrier. However, in the endothelium of human brain tumors expression of PLVAP is specifically up-regulated, making it a suitable antiangiogenic target for brain tumor therapy and cerebral edema. Furthermore, PLVAP is expressed in the vasculature of most other human tumors and as such useful as marker for tumor angiogenesis.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:50 Flow Cytometry: 1:50 Western Blot: 1:50 Immunohistochemistry (Frozen sections): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Isolated hilar vessels from human lymph nodes.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human plasmalemma vesicle associated protein (PLVAP).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Niemelä, H et al; Molecular identification of PAL-E, a widely used endothelial-cell marker. Blood 2005, 106: 3405 2. Carson-Walter, E et al; Plasmalemmal vesicle associated protein-1 is a novel marker implicated in brain tumor angiogenesis. Clin Canc Res 2005, 11: 7643
USBio References
No references available
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