Technical Data

P4256-31G
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Highly Purified
Applications
E
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Plasminogen Activator Inhibitor Type 1 (PAI-1)

Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6).

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:100,000 Optimum dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a colorless solution in 0.05M sodium phosphate, 0.1M sodium chloride, 1mM EDTA, pH 6.6
Purity
≥98%
Specificity
Recognizes Plasminogen Activator Inhibitor Type 1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sherman, P.M. et al. (1992) J Biol Chem 267:7588-7595.|2. McMahon, G. A. et al. (2001) J Biol Chem 276:33964-33968.|3. Devy, L. et al. (2002) FASEB J 16:147-154.|4. Schneider, j. et al. (2003) Brit J Canc 88(1):96-101|5. hou, A. et al. (2003) 10(7):541-544|6). Rezaie, R. et al. (2001) J Biol Chem 276:15567-15570|7. Stegansson, S. et al. (1998) J Biol Chem 273:6358-6366
USBio References
No references available
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