Plk is a serine/threonine kinase which is highly homologous to the Drosophila serine/threonine kinase encoded by the mutant polo. Plk and polo encode proteins of similar size and possess a similar overall structure, with their catalytic domains located near the N-terminal region. In Drosophila, the polo kinase appears to be involved in cell cycle regulation and chromosome segregation, and homozygous mutations in polo results in the appearance of aberrant chromosome segregation and frequently lethal mitosis. Studies on mammalian cells indicate that Plk expression is restricted to proliferating cells. Consistent with this finding, Plk mRNA can be detected in most tissues during mammalian development; however, in adult tissues, its expression is restricted to the thymus and ovaries. Plk is related to several other serine/threonine kinases which include: Saccharomyces cerevisiae CDC5, murine Snk (serum-inducible kinase), murine Fnk (FGF-inducible kinase), and murine Sak (Snk/Plk-akin kinase. Plk protein levels are cell-cycle regulated with low levels of detected in G1 phase and higher levels detected in S and G2-M. Plk kinase activity is also cell-cycle regulated and is modulated, at least in part, by phosphorylation of Plk on one or more serine residues by an as yet unidentified kinase. Phosphorylation on serine serves to stimulate Plk kinase activity and results in slower migration of the Plk protein when analyzed by SDS-PAGE. Interestingly, activation of the Plk kinase activity parallels that of Cdc2; however, Cdc2 kinase is not thought to be directly responsible for Plk phosphorylation. Suitable for use in Immunofluorescence, FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Immunofluorescence: 2-5ug/ml FLISA: 0.1-1ug/ml Western Blot: 1-2ug/ml Immunoprecipitation: 5ug Optimal dilutions to be determined by the researcher. HeLa, A431 and NIH 3T3 cells. Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

A His-tagged Plk fusion protein (C-term Plk1) corresponding to aa402-603 of the human Plk1 protein.

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by Protein A affinity chromatography.

Recognizes the human Plk1 protein at ~68kD. Species Crossreactivity: mouse, rat and Xenopus.