Technical Data

P4276-01B
Clone Type
Polyclonal
Host
Goat
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Ca Hu Mo Rt
Accession #
NP_002660.1
Gene ID
5356, 60376
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-PLRG1 (Pleiotropic Regulator 1, PRL1 Homolog, Arabidopsis, MGC110980, PRP46, PRPF46)

Necessary for spliceosome assembly and for pre-mRNA splicing.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 0.5-2ug/ml, mouse kidney extracts observed on ~60kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PVSWKPEIIKRKRF, from mouse PLRG1, at C-terminal (NP_002660.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse PLRG1. Species sequence homology: Bovine, canine, human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ajuh P, Lamond AI Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1 Nucleic Acids Res. 2003 Nov 1;31(21):6104-16.
USBio References
No references available
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