PML protein is a tripartite motif (TRIM)-containing nuclear protein that may function as, among other things, a transcription factor, a coactivator of nuclear receptors, a regulator of apoptosis, a mediator of interferon-induced antiviral response, and as a suppressor of growth and oncogenic transformation. PML is localized to the nucleoplasm and in distinct subnuclear structures referred to as Promyelocytic Leukemia Bodies (also known as Nuclear Domain 10). Localization of PML to Promyelocytic Leukemia Bodies requires modification of PML protein by the Small Ubiquitin Modifier (SUMO) and is required for proper formation and integrity of these subnuclear structures. At least 14 splice variants of PML ranging in molecular weight from 48-97kD (predicted) have been described in the literature. The functional significance of the various splice variants is not well understood. In patients with Acute Promyelocytic Leukemia, the PML gene is involved in at least two specific chromosomal translocations that result in the expression of chimeric proteins with the Retinoic Acid Receptor alpha. All isoforms of PML, as well as the PML-RARalpha chimeric proteins expressed in Type A and Type B APL contain an identical N-terminus but vary in the C-terminal portion of the protein.

Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Western Blot: 0.5-1ug/ml of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

6His fusion protein corresponding to aa1-581 of mouse PML (promyelocytic leukemia protein).

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by Protein G affinity chromatography.

Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not crossreact with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4296. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.