Rabbit Anti-Pokemon (POK Erythroid Myeloid Ontogenic Factor, Factor that Binds to Inducer of Short Transcripts Protein 1, Factor Binding IST Protein 1, FBI1, FBI-1, HIV-1 1st-binding Protein 1, Leukemia/lymphoma-related Factor, LRF, TTF-I Interacting Peptide 21, TIP21, Zinc Finger and BTB Domain-containing Protein 7A, ZBTB7A, ZBTB7) (Not for Export EU)
Pokemon is a 584aa protein containing 1 BTB (POZ) domain and 4 C2H2-type zinc fingers. It is involved in transcriptional regulation and influencing the switch from cellular proliferation to terminal differentiation. It is a cellular factor that preferentially associates with active chromatin and that can specifically stimulate Tat-activated HIV-1 transcription. Pokemon expression levels have been shown to predict biological behavior and clinical outcome.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
MCF7 cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa1-50 of human Pokemon.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity
Recognizes human Pokemon. Species Crossreactivity: chicken, mouse, opossum, rat.
