Technical Data

P4448-45
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Accession #
NP_955452.3
Gene ID
10721
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)

Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA (Peptide): 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to DTHQQLTCSNDES, from the internal region of human DNA polymerase theta
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human DNA polymerase theta

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kawamura K, Bahar R, Seimiya M, Chiyo M, Wada A, Okada S, Hatano M, Tokuhisa T, Kimura H, Watanabe S, Honda I, Sakiyama S, Tagawa M, O-Wang J. DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers. Int J Cancer. 2004 Mar;109(1):9-16.
USBio References
No references available
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