Mouse Anti-PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1)
PTBP1 is also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57kD RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5kb.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2ul/ml Immunohistochemistry: 15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to full length human PTBP1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human PTBP1.
