Mouse Anti-pan-MAGUK (Postsynaptic Density Protein 95, PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)
Disks large homolog 4, FLJ97752, FLJ98574, Postsynaptic density protein 95, PSD95, PSD-95, SAP90, SAP-90, Synapse-associated protein 90
The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1ug/ml detects members of the MAGUK protein family on 10ug of rat brain tissue lysate. Immunohistochemistry (paraffin): 1:500 detects the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues. Optimal dilutions to be determined by the researcher.
Positive Control
Rat brain tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.
Form
Supplied as a liquid in Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human pan-MAGUK. Species crossreactivity: rat. Species sequence homology: mouse, 100%. Exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility, http://neuromab.ucdavis.edu)
