Technical Data

P5250-06A
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Ca Hu Rt
Accession #
NP_055167.2
Gene ID
25833
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-POU2F3 (POU Domain, Class 2, Transcription Factor 3, Octamer-binding Protein 11, Oct-11, Octamer-binding Transcription Factor 11, OTF-11, Transcription Factor PLA-1, Transcription Factor Skn-1, OTF11, PLA1)

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 1-3ug/ml, lysates of cell lines HEK293 and HepG2 observed on ~50kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-EPHLEASQHLPVPKH, from human POU2F3, at the internal region of the protein (NP_055167.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human POU2F3. Species sequence homology: Bovine, canine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kukimoto I, Mori S, Sato H, Takeuchi T, Kanda T, Transcription factor human Skn-1a enhances replication of human papillomavirus DNA through the direct binding to two sites near the viral replication origin. The FEBS journal 2008 Jun 275(12):3123-35.
USBio References
No references available
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