Technical Data

P5250-23F
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Accession #
NP_006227.1
Gene ID
5455, 18993, 192109
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-POU3F3 (POU Domain, Class 3, Transcription Factor 3, Brain-specific Homeobox/POU Domain Protein 1, Brain-1, Brn-1, Octamer-binding Protein 8, Oct-8, Octamer-binding Transcription Factor 8, OTF-8, BRN1, OTF8)

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 1-3ug/ml, observed in mouse and rat spinal cord lysates on ~48kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-HMLSHAHQWVTAL, from POU3F3, at the internal region of the protein (NP_006227.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes POU3F3. Species Crossreactivity: Mouse and rat. Species sequence homology: Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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