Technical Data

P5505-06B
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
IP WB
Crossreactivity
Hu Mo
Gene ID
5468
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PPARg (Peroxisome Proliferator Activated Receptor, gamma) (Not for Export EU)

Peroxisome proliferator-activated receptor, gamma (PPARgamma) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARg is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARg also modulates insulin sensitivity, cell proliferation and inflammation (3). PPAR gamma transcriptional activity is inhibited by MAP kinase phosphorylation of PPAR gamma at Ser84 (4,5).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the sequence around Asp69 of human PPAR gamma. Species sequence homology: rat.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Serum
Specificity
Recognizes endogenous levels of total human PPAR gamma protein at 53kD (PPARg1) and 57kD (PParg2). Species crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1 Tontonoz, P. et al. (1995) Curr. Opin. Genet. Dev. 5, 571-576. 2 Rosen, E.D. et al. (1999) Mol. Cell 4, 611-617. 3 Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol. Sci. 21, 469-474. 4 Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816. 5 Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132.
USBio References
No references available
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