Mouse Anti-PRAS40 (Proline-Rich AKT Substrate)
Proline-Rich AKT Substrate (PRAS40) is a 40kD substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. AKT and PRAS40 are components of the phosphoinositide 3-kinase (PI3K) pathway. This pathway plays a role in glucose uptake, cell growth and apoptosis inhibition. The precise function of PRAS40 is not yet known; however, it has been hypothesized that PRAS40 interacts with SH3 and WW domain containing proteins and may change the function of these proteins.
Applications
Suitable for use in, Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Immunofluorescence (IC): 1:100-1:500 Optimal dilutions to be determined by the researcher.
Recommended Positive Controls
HeLa cells; Jurkat cells.
Hybridoma
BALB/c mouse splenocytes were fused with FO myeloma cells.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant full length human PRAS40 expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Specificity
Recognizes human PRAS40 at ~40kD. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not crossreact with mouse.
