Goat Anti-PRDM16 (PR Domain Zinc Finger Protein 16, PR Domain-containing Protein 16, Transcription Factor MEL1, MDS1/EVI1-like Gene 1, KIAA1675, MEL1, PFM13)
PRDM16, also known as Mel1, is a zinc finger protein that plays a role in the differentiation of brown adipose tissue, which plays a role in dissipating heat in response to cold or excess feeding. PRDM16 works together with CEBPB to regulate the differentiation of myoblastic precursors into brown adipose cells. PRDM16 is also a repressor of TGF beta signalling, interacting with SKI to promote the recruitment of the SMAD3-HDAC1 complex to the promoter of TGF beta target genes.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TSESGAFHPINHL, from PRDM16, at C-terminal (NP_071397.2, NP_955533.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes PRDM16. Species sequence homology: Human.
