Technical Data

P6300-82A
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
12K165
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Accession #
NP_758844
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Presenilin Enhancer Protein 2 (PEN2, PEN-2, Gamma-secretase Subunit PEN-2, MDS033, PSENEN)

Gamma-secretase subunit PSENEN, also called PEN-2 is a 10kD member of the PEN-2 family. Human PSENEN is 101aa in length, and is a multi-pass transmembrane protein. Residues 1-17 are on the luminal side of the endoplasmic reticulum or Golgi apparatus, where PSENEN is primarily located. aa18-38 form a transmembrane region, and aa39-60 form a cytoplasmic segment. Another transmembrane segment is formed by residues 61-81, and residues 82-101 are located on the luminal side of the ER or cis-Golgi. Human PSENEN shares 96% aa sequence identity with mouse and rat PSENEN. Functionally, PSENEN is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and beta-amyloid precursor protein.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa89-98 from human PSENEN.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human PSENEN.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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