P9004-05B
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PRMT1, CT (K342) (Protein Arginine N-Methyltransferase 1, Interferon Receptor 1-bound Protein 4, HMT2, HRMT1L2, IR1B4)
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology.
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human PRMT1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human PRMT1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Zhang, X., et al., EMBO J. 19(14):3509-3519 (2000). 2. Scorilas, A., et al., Biochem. Biophys. Res. Commun. 278(2):349-359 (2000). 3. Scott, H.S., et al., Genomics 48(3):330-340 (1998). 4. Abramovich, C., et al., EMBO J. 16(2):260-266 (1997). 5. Nikawa, J., et al., Gene 171(1):107-111 (1996).USBio References
No references available