Goat Anti-PIP (BPNT1, 3'(2'),5'-bisphosphate Nucleotidase 1, Bisphosphate 3'-nucleotidase 1, PAP-inositol-1,4-phosphatase)
PIP belongs to the inositol monophosphatase family. It hydrolyses adenosine 3'phosphate 5'-phosphosulfate to adenosine 5-phosphosulfate and 3'(2)'-phosphoadenosine 5'-phospate to AMP. It requires magnesium as a cofactor. Lithium is an uncompetive inhibitor and inositol 1,4-bisphosphate is a competitive inhibitor of PIP. Lithium is one of the drugs used to treat manic depression and the inhibition of PIP may account for lithium-induced nephrotoxicity.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 1-3ug/ml, observed in human liver lysates on ~37kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RNYDYYASRVPESIK, from human BPNT1, at C-terminal (NP_006076.4).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human BPNT1. Species sequence homology: Mouse and rat.
