Mouse Anti-Proprotein Convertase 2 (PC2, KEX2 Like Endoprotease 2, KEX2-like Endoprotease 2, Neuroendocrine Convertase 2, NEC 2, NEC2, Prohormone Convertase 2, Proprotein Convertase Subtilisin/kexin Type 2, PC2, PCSK2, SPC2)
Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic aa residues (1). Autoactivation of PCSK2 occurs in a post-Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml Immunoprecipitation: 25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa110-638 from Proprotein Convertase 2/PCSK2 expressed in CHO cells (P16519).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human PCSK2. Species sequence homology: mouse (97%). No cross-reactivity with recombinant human Proprotein Convertase 1, 7, 9, or Furin.
