Technical Data

P9052
Grade
Molecular Biology Grade
Applications
FC
Shipping Temp
RT
Storage Temp
4°C
Anti-Propidium Iodide Solution, 1mg/ml
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium Iodide; 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium Iodide Methiodide; [3-(3,8-Diamino-6-phenyl-5-phenanthridinio)propyl]diethylmethylammonium Diiodide; 3,8-Diamino-5-(diethylmethylaminopropyl)-6-phenylphenanthridinium Diiodide; Propidium Diiodide

Propidium iodide (PI) is a fluorescent dye [FW 668.41] that binds specifically to DNA. This property has led to its common use in evaluation of cell cycle, aneuploidy and apoptosis by flow cytometry. When excited by a laser light at 488 nm, PI emits a signal that can be monitored by the red wavelength detector typically reserved for phycoerythrin (usually FL2). Propidium iodide is excluded from viable cells, thus analysis of DNA content in cells requires membrane permeabilization. In evaluation of apoptosis, PI is used to distinguish necrotic cells or cells in late stages of apoptosis, which have permeabilized membranes and thus bind PI, from viable cells or cells in early apoptosis where membranes are intact and PI is excluded.

Solubility (1mg/ml)
Red, clear and complete
Application
Used in conjunction with the United States Biological Annexin V Apoptosis Detection Kits.(Catalog no.s A 2296-10, -20, -30)
Storage and Stability
Store at 4°C. Protect from light
Concentration
~1mg/ml
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net