Rabbit Anti-PSMB1, CT (Proteasome gamma Chain, Multicatalytic Endopeptidase Complex Subunit C5, Macropain Subunit C5, Proteasome Component C5, Proteasome Subunit beta Type-1, PSC5)
PSMB1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
Applications
Suitable for use in ELISA, Indirect Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Indirect Flow Cytometry: 1:10-1:50 Immunohistochemistry: 1:50-1:100 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa212-241 from the C-terminal region of human PSMB1.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human PSMB1. Species Crossreactivity: mouse.
