Technical Data

P9102-39H
Clone Type
Monoclonal
Host
Rat
Source
Human
Isotype
IgG1
Clone Number
RCR-252
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Protein C Receptor, Endothelial (EPCR, Activated Protein C Receptor, APC Receptor, APCR, CCCA, CCD41, CD201, CD201 Antigen, Centrocyclin, MGC23024, PROCR)

The protein C anticoagulant pathway serves as a major system for controlling thrombosis, limiting inflammatory responses, and potentially decreasing endothelial cell apoptosis in response to inflammatory cytokines and ischemia. The essential components of the pathway involve thrombin, thrombomodulin, the endothelial cell protein C receptor (EPCR), protein C and protein S. The pathway is initiated when thrombin binds to thrombomodulin on the surface of endothelium. EPCR augments protein C activation by binding protein C and presenting it to the thrombin-thrombomodulin activation complex. Activated protein C (APC) retains its ability to bind EPCR, and this complex appears to be involved in some of the cellular signalling mechanisms that down-regulate inflammatory cytokine formation (TNF, IL-6). EPCR is shed from the vasculature by inflammatory mediators and thrombin. EPCS binds to activated neutrophils in a process that involves proteinase 3 and Mac-1. EPCR can undergo translocation from the plasma membrane to the nucleus.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Inhibition of biological activity. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:10 Immunohistochemistry (frozen): 1:10 For inhibition of biological activity in vitro a dilution of 1:5 is advised as start concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Tail base human EPCR-positive RE-1 cells
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human EPCR. Inhibits thrombin-dependent protein C activation and blocks APC-mediated signalling.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Domotor, E et al; Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1. Blood 2003, 101: 4797 2. Sturn, D et al; Expression and function of the endothelial protein C receptor in human neutrophils. Blood 2003, 102: 1499 3. Ye, X et al; The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in arteries, veins, and capillaries. Biochem biophys res commun 1999, 259: 671
USBio References
No references available
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