Technical Data

P9103-25V2
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PRKCG, CT (Protein Kinase C, gamma type, PKC-gamma, PKCG)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 305-336 in the C-terminal region of human PRKCG, UniProt Accession #P05129. Species sequence homology: monkey
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human PKC. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Uchino, M., et al., J. Biol. Chem. 279(3):2254-2261 (2004). 2.van de Warrenburg, B.P., et al., Neurology 61(12):1760-1765 (2003). 3.Yabe, I., et al., Arch. Neurol. 60(12):1749-1751 (2003). 4.Ananthanarayanan, B., et al., J. Biol. Chem. 278(47):46886-46894 (2003). 5.Correia, S.S., et al., J. Biol. Chem. 278(8):6307-6313 (2003).
USBio References
No references available
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