P9103-31B-APC
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
APCIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IF IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeRabbit Anti-PKC nu, CT (Serine/Threonine-protein Kinase D3, Protein Kinase C, nu Type, nPKC-nu, Protein Kinase EPK2, PRKD3, PRKCN, EPK2) (APC)
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
Applications
Suitable for use in FLISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 860-890 in the C-terminal region of human PKC nu. Species Sequence Homology: mouse
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A/G affinity chromatography
Specificity
Recognizes human PKC nu.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004). 2. Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003). 3. Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003). 4. Bennasser, Y., et al., Virology 303(1):174-180 (2002). 5. Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).USBio References
No references available