Technical Data

P9103-68B
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PKC theta, CT (Protein Kinase C theta Type, nPKC-theta, PRKCQ, PRKCT)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 318-348 in the C-terminal region of human PKC theta, UniProt Accession #Q04759.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human PKC theta.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Carrasco, S., et al., Mol. Biol. Cell 15(6):2932-2942 (2004). 2.Sun, L., et al., Blood 103(3):948-954 (2004). 3.Burchfield, J.G., et al., J. Biol. Chem. 279(18):18623-18632 (2004). 4.Ishaq, M., et al., J. Biol. Chem. 278(41):39296-39302 (2003). 5.Cipriani, B., et al., J. Immunol. 169(10):5761-5770 (2002).
USBio References
No references available
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