Rabbit Anti-PKN1 (Serine/Threonine-protein Kinase N1, Protease-activated Kinase 1, AK-1, Protein Kinase C-like 1, Protein Kinase C-like PKN, Protein Kinase PKN-alpha, Protein-kinase C-related Kinase 1, PAK1, PKN, PRK1, PRKCL1, Serine-threonine Protein Kinase N, ) (Not for Export EU)
Protein Kinase 1 (PKN) belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000 Immunoprecipitation: 1:50-1:200 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.
Positive Control
Spleen, lymphoid, CA cell lines
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa911-929 of human PKN (EAPTLSPPRDARPLTAAEQ).
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity
Recognizes human PKN. Species Crossreactivity: canine, mouse, rat.
