Technical Data

P9103-80
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PRKG2, CT (cGMP-dependent Protein Kinase 2, cGK 2, cGK2, cGMP-dependent Protein Kinase II, cGKII, PRKGR2)

cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human cGKII (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human cGKII.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Vaandrager, A.B., et al., J. Biol. Chem. 278(31):28651-28658 (2003). 2.Gambaryan, S., et al., Biochem. Biophys. Res. Commun. 293(5):1438-1444 (2002). 3.Orstavik, S., et al., Biochem. Biophys. Res. Commun. 220(3):759-765 (1996). 4.Fujii, M., et al., FEBS Lett. 375(3):263-267 (1995). 5.Hema Rangaswami, et al. J. Biol. Chem., Vol. 284, Issue 22, 14796-14808. (Applications: WB).
USBio References
No references available
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