Mouse Anti-PPP2R4 (Serine/Threonine-protein Phosphatase 2A Activator, PP2A, Subunit B', PR53 Isoform, Phosphotyrosyl Phosphatase Activator, PTPA, Serine/Threonine-protein Phosphatase 2A Regulatory Subunit 4, Serine/Threonine-protein Phosphatase 2A Regulatory Subunit B', PTPA)
Serine/threonine protein phosphatase 2A regulatory subunit 4 (PPP2R4) is one of the constituents of the regulatory subunit B' of PP2A (1). PPP2R4 is also known as the phosphatase activator (PTPA) and known to have the peptidyl prolyl isomerase (PPIase) activity (2-3). PPIases catalyze cis-trans isomerization of peptide bonds of proline residues in the polypeptide chain; this leads to the rapid folding of proteins (2). The ATPase activity of PPP2R4 is required for the phosphotyrosyl phosphatase activity of PP2A (2-4). Evidence suggests that PPP2R4 induces apoptosis independent of the canonical PP2A-mediated pathways (4).
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein specific to the mouse PPP2R4.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of total mouse PPP2R4. Species Crossreactivity: Human and monkey. Does not cross-react with other PP2A proteins.
