Mouse Anti-Protein S
Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70kD and consists of a single polypeptide chain. The concentration of protein S in human plasma is 25ug/ml, with a half-life of approximately two days. About 40% of protein S in plasma is in a free form, wheraes 60% is complexed with C4b-binding Protein (C4BP). Only the free protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa.
Applications
Suitable for use in ELISA. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653: Female CF1 Balb/c mice immunized by intraperitoneal injection.
Culture Medium
RPMI 1640 with 10% fetal calf serum.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Protein S isolated from human plasma.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Protein S. Reacts strongly with Protein S. Strong reaction is seen in sandwich ELISA in combination with a biotin-labeled antibody against protein S. Reactivity is Ca2+ dependent and is optimal when using Tris buffer containing 1mM calcium chloride. In Dot Blot, SDS-treatment abolishes the reactivity.
