Technical Data

P9108-48-ML550
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™550
Isotype
IgG1
Clone Number
2B2.21 (EIF2a)
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-Protein Synthesis Initiation Factor 2 alpha (Eukaryotic Translation Initiation Factor 2 alpha, Eukaryotic Translation Initiation Factor 2 Subunit 1 alpha 35kDa, eIF2 alpha, eIF2alpha, eIF-2-alpha, eIF-2alpha, eIF 2a, eIF2a, eIF-2a, CDA02, eIF2S1) (MaxLight 550)
Eukaryotic Translation Initiation Factor 2 Subunit alpha

eIF-2a is a 36kD protein and is ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. The phosphorylation of eIF-2a is an important regulatory process in protein synthesis. In mammalian cells, eIF-2a is phosphorylated at serine 51 by at least two kinases: the heme-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2a blocks the GDP-GTP exchange activity of eIF-2b, resulting in the suppression of protein synthesis.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Human Jurkat, CEM and HeLa cells, mouse 3T3L1 and rat PC-12 cells.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human eIF-2a
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Purity
Purified by Protein A/G affinity chromatography from ascites.
Specificity
Recognizes the a subunit of human eukaryotic translation initiation factor 2 (eIF-2a) at ~36kD. Species Crossreactivity: Mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Datta, B., et al., Exp. Cell Res. 250: 223-230 (1999). 2. Ernst, H., et al., J. Biol. Chem. 262(3): 1206-1212 (1987). 3. Lu, J., et al., J. Biol. Chem. 274: 32,198-32,203 (1999). 4. Kimball, S.R., Int. J. Biochem. Cell Biol. 31: 25-29 (1999). 5. Kimball, S.R., et al., Biochim. Biophys. Acta 1201: 473-481 (1994). 6. Pavitt, G.D., et al., Genes Dev. 12: 514-526 (1998). 7. Patel, C.V., et al., J. Biol. Chem. 275: 37,993-37,998 (2000). 8. Zhang, P.C., et al., 22: 3864-3874 (2002).
USBio References
No references available
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