P9109-17H
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Hu MoShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Protein Tyrosine Phosphatase 1B (PTP1B, PTP-1B, Protein Tyrosine Phosphatase Non-receptor Type 1, PTPN1, Tyrosine-protein Phosphatase Non-receptor Type 1)
Protein-tyrosine Phosphatase 1B, EC=3.1.3.48
PTP1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
ELISA: 0.25ug/ml Western Blot: 0.5-2.5ug/ml detects a band at ~50kD. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to amino aa 1-321 of human PTP1B.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PTB1B. Species crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Ragab, A., et al., J. Biol. Chem. 278(42):40923-40932 (2003). 2. Sun, J.P., et al., J. Biol. Chem. 278(14):12406-12414 (2003). 3.Boute, N., et al., EMBO Rep. 4(3):313-319 (2003). 4. Li, S., et al., Arch. Biochem. Biophys. 410(2):269-279 (2003). 5. Yigzaw, Y., et al., J. Biol. Chem. 278(1):284-288 (2003).USBio References
No references available