Technical Data

P9183-01D
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_005020.1
Gene ID
5309
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-PITX3 (Pituitary Homeobox 3, Paired-like Homeodomain Transcription Factor 3, Homeobox Protein PITX3, PTX3)

PITX3 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Western Blot: 0.3-1ug/ml, observed in human brain (cerebellum and cerebral cortex) lysates on ~40kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to PEHGCKGQEHSD, from human PITX3, at the internal region of the protein (NP_005020.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PITX3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kim J, Inoue K, Ishii J, Vanti WB, Voronov SV, Murchison E, Hannon G, Abeliovich A. A MicroRNA feedback circuit in midbrain dopamine neurons. Science. 2007 Aug 31;317(5842):1220-4.
USBio References
No references available
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