P9544-01B
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_003468, O00330Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PDX1, CT (Pyruvate Dehydrogenase Protein X Component, Mitochondrial, Dihydrolipoamide Dehydrogenase-binding Protein of Pyruvate Dehydrogenase Complex, Lipoyl-containing Pyruvate Dehydrogenase Complex Component X, E3-binding Protein, E3BP, PDHX)
PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from selected from the C-terminal region of human PDX1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human PDX1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Ling, M., et al., Hum. Mol. Genet. 7(3):501-505 (1998). 2. Harris, R.A., et al., J. Biol. Chem. 272(32):19746-19751 (1997). 3. Murray, J., et al., FEBS Lett. 529(2-3), 173-178 (2002).USBio References
No references available