P9580-20
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IP WBCrossreactivity
Bo Hu Mo RtGene ID
9019Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PZR, Phosphorylated (Tyr263) (Myelin Protein Zero-like Protein 1, Protein Zero-related, MPZL1, UNQ849/PRO1787)
PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is a main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. Recently, PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan Syndrome (8).
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Tyr263 of human PZR.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human PZR when phosphorylated at Tyr263. Species Crossreactivity: Bovine, mouse and rat. Does not react with other tyrosine kinase proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72. 2.Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9. 3.Kusano, K. et al. Endothelium 15, 127-36. 4.Zhao, R. et al. (2002) J Biol Chem 277, 7882-8. 5.Zhao, R. et al. (2003) J Biol Chem 278, 42893-8. 6.Zannettino, A.C. et al. (2003) Biochem J 370, 537-49. 7.Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33. 8.Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.USBio References
No references available