Technical Data

R0009-87B
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_071735.2
Gene ID
64145
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-Rabenosyn 5 (Rabenosyn-5, 110kD Protein, FLJ34993, FYVE Finger-containing Rab5 Effector Protein Rabenosyn-5, MGC126210, Zinc Finger FYVE Domain-containing Protein 20, ZFYVE20)

DNA binding protein involved in regulation of transcription.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 0.3-1.5ug/ml, observed in A431, fibroblast (epidermoid carcinoma) lysates on ~110kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-ELKHTLAKQKGGTD, from human ZFYVE20, at C-terminal (NP_071735.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ZFYVE20.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000 Oct 30;151(3):601-12.
USBio References
No references available
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