R1032-10
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)Rabbit Anti-RAIDD, IN (RIP-associated ICH-1/CED-3-homologous protein with DD, CRADD, Caspase and RIP adapter with DD)
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD). RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.
Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
Whole cell lysate from HeLa or K562 cells can be used and a 22kD band should be detected.
Storage and Stability
Stored at 4°C. Stable for one year.
Immunogen
Peptide corresponding to amino acids 99-117 of human RAIDD (1).
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human RAIDD (IN) CRADD
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Duan, H., Dixit, V.M., Nature, 385:86-89 (1997). 2. Ahmad, M., et al., Cancer Res., 57:615-619 (1997). 3. Hofmann, K., et al., Trends Biochem Sci., 22:155-156.USBio References
No references available